FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42793920
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG521000.12174626675939013No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA500090.11686005862515049No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG448180.10472983077969955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG155100.017.00754245
GGCATAG166650.015.92079235
GTCACTC255500.014.6886628
GGTCACT263600.014.2290927
ACCCGGC98600.013.9507855
AGGTCAC278900.013.803316
GAGCTTA196450.013.71413644
CATAGGG196350.013.66010937
ATAGGGG197200.013.62269838
ACTCTCC277450.013.46976711
GAGGTCA290650.013.4092015
GGGAGGT315200.013.2994763
TGGCATA204700.013.03844334
ACGGACA108300.012.88531118
TCACTCT299400.012.7075499
GGAGGTC306550.012.50134854
CGGACAG108100.012.49339719
GCATAGG215150.012.45622936
ATAGCTC102550.012.38525839
TCACCCG112400.012.2984593
Produced by FastQC (version 0.11.5)