FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53206087
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT900260.16920244482553284No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG618790.11630060297424241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG79100.029.128196
GTTCGAC82850.027.6194085
TCGACGA88200.026.1736767
CGGTATA113350.026.12246720
CGACGAG86050.025.834028
AGTTCGA104300.022.2197344
ATGACGG135150.020.79313916
ACGAGGA138150.020.35846710
GACGAGG137550.020.3164089
AGACCGC242550.020.2320694
GACCGCG240650.020.1487145
ACCGCGT244200.020.0218436
GAACGAT250550.019.9358345
CAGTTCG120400.019.7526913
CAGACCG253550.019.4781953
GTATAGA163700.019.36613722
CGAGGAT135300.019.14058911
CCGCGTT255300.019.1158967
GACGGTA154900.019.1151718
CGCGTTC262700.018.6116818
Produced by FastQC (version 0.11.5)