FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54566093
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG942320.17269332440568907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG253650.017.20509745
GTTGTCG31700.016.6781426
GGCATAG286400.015.2158335
ATAGGGG301250.014.57032538
GTCACTC429600.014.52128
GAGCTTA303650.014.46413644
GGTCACT434250.014.2826437
TGGCATA312850.014.04447534
GGGAGGT470100.014.0010113
AGGTCAC454350.013.9374096
GGAGGTC465100.013.6535394
ACTCTCC461600.013.63142811
GAGGTCA482500.013.254455
CATAGGG340150.012.84451937
TGTCGTT43700.012.66464828
TCACTCT517600.012.3175949
CACTCTC512150.012.22456710
GCATAGG359800.012.11802136
TGGGAGG385550.011.9761622
ACCCGGC95650.011.5513165
Produced by FastQC (version 0.11.5)