FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55998730
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1001450.1788344128518629No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC569450.101689806179533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT209100.023.8497876
AGACCGC215950.023.1701934
CCGCGTT216150.023.061497
GACCGCG216550.023.0332035
GAACGAT221950.022.52516745
CGCGTTC223750.022.3384918
CAGACCG225750.022.234183
TTCGACG22000.020.0403946
TCAGACC258650.019.7380312
AGAACGA257700.019.46746644
ATACGGA256800.019.42510433
TCGGCGC139400.019.3524345
AATACGG260350.019.17791232
TTCGGCG144050.019.02186244
TACGGAG264500.018.90165334
GCGTTCT272100.018.4931249
ACGTTCG31650.017.8390966
AACGCTA131650.017.43109523
ACGCTAC135850.016.9418724
ACGGAGA297800.016.78744135
Produced by FastQC (version 0.11.5)