FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55998730
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG569430.10168623466996482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC119900.015.915115
AGCTTAG180400.015.65018345
ACGGACA131800.014.38890118
GGCATAG198450.014.17022435
TCACCCG142850.014.1136023
ATAGCTC131550.013.749413539
CGGACAC137900.013.56137913
ACACGGA140150.013.228353516
CATAGGG215250.013.18940137
GTCACTC285500.013.1558618
CGGACAG142450.013.07603219
GAGCTTA222700.012.75089644
GGTCACT290300.012.6429057
ATAGGGG229550.012.42684638
GCATAGG227700.012.418920536
CGTCGAT44050.012.36348212
CACCCGG161250.012.3493224
CCGGACA158400.012.21840812
GACACGG151650.012.21237515
TGGCATA233350.012.1958334
Produced by FastQC (version 0.11.5)