FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49122358
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT771840.15712600767251442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC161800.023.6924824
GACCGCG166850.022.9345425
ACCGCGT168300.022.8173126
CAGACCG174650.022.1811493
GAACGAT171900.021.9607945
CCGCGTT180400.021.2744127
CGCGTTC188450.020.4134068
TTCGACG20150.018.9796836
AGAACGA205950.018.52772144
ATACGGA206350.018.26925733
AATACGG207600.018.23519732
TACGGAG207500.018.14575634
TCAGACC219700.018.0839922
GCGTTCT220800.017.4633739
CTCAGAC273500.016.4868681
CAATACG235400.016.27304831
AAGAACG234350.016.18682343
ACGGAGA240950.015.72873635
CCCAATA253850.015.223474529
AACGCTA125150.015.10077823
Produced by FastQC (version 0.11.5)