FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49122358
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG150400.015.13138945
GTTGTCG31100.014.47208226
GGCATAG159350.014.25103235
ATAGGGG166950.013.7896638
CATAGGG175450.012.98049537
GAGCTTA178750.012.79452944
GTCACTC237400.012.5237818
GCATAGG185350.012.36010936
TGGCATA185650.012.25681334
GGTCACT244750.012.1110427
GGGAGGT279950.011.9627543
TGTCGTT39100.011.47429628
ACTCTCC264300.011.40192611
AGGTCAC274100.011.3964526
ATATAGC46500.011.2607376
GGAGGTC282600.010.9914174
GCTAAGT45700.010.22808214
GAGGTCA316550.010.12478355
ACCCGGC82500.09.970935
GTCGTTC46800.09.73207329
Produced by FastQC (version 0.11.5)