FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50327613
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT93300.018.1567296
AGACCGC94150.018.02894
GACCGCG96950.017.508215
TTCGACG20000.016.8727366
CCGCGTT100950.016.8030957
CCCGTAT32300.016.244241
CGCGTTC108400.015.6897788
CAGACCG110450.015.5516683
GTCCGTT38800.014.8577921
TCGGCGC63800.013.93757745
ACGGTAC73900.013.83000245
TTCGGCG66450.013.74541744
CTACACG47500.013.64927945
TACGGTA76600.013.33379944
CCTACTC79450.013.02532618
ACGTTCG20000.012.9357646
GTTCCTA78350.012.92104815
TCGACGA27250.012.7964497
CTCCGTT69700.012.7617521
CGCTACT62600.012.75943225
Produced by FastQC (version 0.11.5)