FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54385200
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1244150.22876628200319207No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1002690.18436817369431388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG31950.024.2886076
AGACCGC345850.019.7600784
TCGACGA40350.019.5108267
GAACGAT342400.019.43072145
ACCGCGT348300.019.4257936
GACCGCG346950.019.4251355
CGACGAG40700.019.0663998
CCGCGTT359950.018.8156727
CAGACCG370300.018.5403673
CGCGTTC365700.018.5256738
GTTCGAC43450.017.9650675
GCCGGCT314450.017.648391
CCGGCTT359050.017.4359572
CGGTATA56500.017.32062120
TCAGACC410300.017.2701662
TTCACGC359700.017.115347
TCACGCT361200.016.9941648
GCGTTCT402800.016.9030599
ACGCTAC360800.016.30046824
AACGCTA361950.016.2656523
Produced by FastQC (version 0.11.5)