FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54385200
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA688940.1266778461787398No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG590870.10864536675419048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG40450.013.62673826
ATATAGC51700.012.7504356
GGGAGGT316600.012.3723043
GGAGGTC301550.012.2809844
AGCTTAG236950.011.8815545
ATAGGGG253400.011.31178438
GGCATAG250200.011.19488335
TGTCGTT52650.010.98157628
TAGCGTA10450.010.76519837
GTCGTTC56100.010.38644329
GAGCTTA276700.010.3048144
ACTCTCC351850.010.21361511
TGGCATA280800.010.03888534
GGTCACT364000.010.0130497
GTCACTC363150.09.9941848
GCTAAGT58450.09.70170714
TCGTTCC60050.09.66577330
TCAGACG106900.09.5130692
ACCCGGC72250.09.4044145
TATAGCT67800.09.2913977
Produced by FastQC (version 0.11.5)