FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42350977
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC78400.014.4060829
AGCTTAG92050.013.34587545
CGGGACG80350.013.27273617
ACCCGGC76000.012.8486785
ACGTCTC88850.012.307384521
TCACCCG84400.012.2368463
ATAGCTC74600.012.21486839
CAGCGCT96150.012.1444068
ACGGACA77750.012.12539618
GGCATAG101300.011.94960635
GTTGTCG16300.011.73272126
TCGGGAC93650.011.65203916
TCTCGGG93950.011.542973514
CGCTCTC98850.011.12982511
CCGGACA89800.011.0739912
GGGACGT100700.010.85898218
GAGCTTA115850.010.81792644
TAGCTCT84900.010.78597340
CATAGGG117650.010.74793837
GACGTCT103100.010.73732120
Produced by FastQC (version 0.11.5)