FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53157891
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC132650.018.9629574
ACCGCGT133750.018.8238226
GACCGCG137500.018.277725
CAGACCG139300.018.235363
GAACGAT138100.017.97141845
CCGCGTT143500.017.5291397
TCGGCGC97900.017.4215645
TGCGCGT57200.017.38708545
CGCGTTC149000.016.8820468
TTCGGCG107900.016.05718644
ACGTTCG36200.015.91126
TTCGACG27550.015.4351816
TACCTAC81150.015.41594135
ATGCGCG65600.015.09207544
ATACGGA168350.014.99537833
ACGGTAC131050.014.88615845
AATACGG169900.014.77911632
AGAACGA171050.014.74628544
TACGGAG174750.014.49769234
CCCGTAT31300.014.3837891
Produced by FastQC (version 0.11.5)