FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49021314
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33250.026.1825626
CGGTATA55600.022.00892320
TCGACGA42100.021.2658047
ACCGCGT126550.020.2289316
GACCGCG126700.020.1390765
GTTCGAC43100.019.9429425
CGACGAG43000.019.8790478
AGACCGC128750.019.853374
CAGACCG142150.018.3934153
CCGCGTT141650.018.0720147
CGCGTTC144650.017.6971878
TCACGCT217250.016.0077448
ACGCTAC208100.016.00132624
AACGCTA208200.015.99290823
TTCACGC222150.015.8268147
CCGGCTT200000.015.5031732
GAACGAT166200.015.36173945
CGCTACC216400.015.35863125
ATGACGG73250.015.29312916
GCCGGCT185500.015.2422071
Produced by FastQC (version 0.11.5)