FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46100971
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT81600.020.2907666
AGACCGC86200.019.3652234
GAACGAT87950.019.11543545
GACCGCG91050.018.2589285
TGCGCGT43600.018.09100545
CCGCGTT91550.018.085497
CAGACCG95150.017.7576983
ACGTTCG26400.017.6391326
AACGCTA104550.016.85202823
ACGCTAC107650.016.40854524
ATGCGCG47700.016.3688644
TCACGCT119300.015.7266568
AATACGG107350.015.57503432
GTTAGTT120450.015.54260744
CGCGTTC107250.015.5219128
AGAACGA110750.015.42074744
CGCTACC115100.015.32717925
ATACGGA109850.015.28202833
TCGGCGC78450.014.99542445
GTCCGTT31350.014.9323571
Produced by FastQC (version 0.11.5)