FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52943351
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4241400.8011204277568301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137900.013.50921945
ATAGGGG141250.013.37697238
GGCATAG143050.012.95989435
GTTGTCG34850.012.58862326
CATAGGG162150.011.62528337
GAGCTTA160950.011.60300144
GGGAGGT241800.011.0491883
AACTCTA122450.011.02444345
GCATAGG171800.010.96079636
GGAGGTC235450.010.7733614
TGGCATA178600.010.53106934
TAATCGG39450.010.4407114
ATATAGC51550.010.3015796
CACCGAA193150.010.04099545
AGGTCAC259300.09.9362716
GTCACTC250850.09.9295798
TGTCGTT47000.09.66886528
GAGGTCA270750.09.6429465
GCACCGA200600.09.55634744
ACTCTCC260450.09.5449311
Produced by FastQC (version 0.11.5)