FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48865076
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG512210.10482128381423166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC125400.017.1015785
AGCTTAG164250.016.22739845
GGCATAG165400.016.01373135
TCACCCG139850.015.611253
CGGACAC140750.015.04577613
ACGGACA140700.015.017838518
CCGGACA143300.014.902925512
GTCACTC231450.014.7585248
CACCCGG150750.014.4824764
CGGACAG148350.014.44094419
GTTGTCG28850.014.27312126
GGTCACT237350.014.2399257
CATAGGG188050.014.13263637
GAGCTTA192400.014.02821644
ACACGGA153850.013.76439216
GCATAGG194550.013.71816636
TGGCATA197150.013.64063934
AGGTCAC258250.013.4770846
ATAGCTC155450.013.23098939
GCCCGGA168250.013.200810
Produced by FastQC (version 0.11.5)