FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42218172
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT37650.021.51340545
TTCGACG19050.019.7239136
ACGTTCG27600.019.2386466
ATGCGCG46000.017.60825244
AACGCTA58850.017.0514823
GTCCGTT30600.016.8557171
ACGGTAC101050.016.47657845
ACGCTAC61450.016.47647324
TCGACGA23300.016.0296387
TTCGGCG68150.015.45082144
CGCTACC65650.015.31956525
TCGGCGC66950.015.22366445
CTCGGTT79850.015.2036221
AGACGCT147300.015.13709441
TACGGTA108650.015.05484544
CCCGTAT26250.014.9297711
GTCGGTT38450.014.82031251
TATTGCG25300.014.58461242
CGACGAG25850.014.44837958
CCTTACG115000.014.43876741
Produced by FastQC (version 0.11.5)