FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53825287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1482030.27534084490808197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT258400.027.22697845
AGAACGA317400.022.28619244
AGACCGC319800.022.027714
ACCGCGT323100.021.83056
GACCGCG323650.021.7309765
CCGCGTT336250.020.9567157
CAGACCG339450.020.851843
CGCGTTC343350.020.5495288
ATACGGA367800.019.09182433
TACGGAG368450.019.02765834
AATACGG371900.018.86926732
TCAGACC381050.018.8347852
GCGTTCT378500.018.7004769
AAGAACG383350.018.5381743
CAATACG399500.017.64437731
ACGGAGA399550.017.58032435
CTCAGAC451550.017.0247421
CCCAATA423650.016.74461229
CACTCCC447800.015.96697724
CGGAGAG443400.015.89744836
Produced by FastQC (version 0.11.5)