FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53825287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4149840.7709833484027684No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG873180.16222486653903026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG239200.018.68717645
GGCATAG272300.016.49346235
ATAGGGG286350.015.78487938
GAGCTTA284450.015.74543644
GTCACTC401200.015.583928
GGTCACT404400.015.5054247
AGGTCAC430300.014.9113566
TGGCATA304500.014.85958334
CATAGGG310900.014.63989637
GGGAGGT478350.014.2634283
ACTCTCC447250.014.25328311
GAGGTCA462250.014.0261485
GGAGGTC466900.013.8381794
GCATAGG329050.013.78478836
GTTGTCG28350.013.14376726
TCACTCT496050.012.7844319
CACCCGG159550.012.4865671
AGCATAG145650.012.36224845
TAGGGGA505850.012.3602439
ACCCGGG156950.012.2012472
Produced by FastQC (version 0.11.5)