FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50749686
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1038400.2046121034128172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG284100.018.39173745
GGCATAG326350.016.0205335
GAGCTTA340500.015.43788944
ATAGGGG346250.015.21674938
GGGAGGT461050.015.0601013
GTCACTC447400.014.9495848
GGAGGTC449600.014.9037044
GGTCACT445300.014.8983667
TGGCATA352200.014.88940234
ACTCTCC457600.014.61143811
CATAGGG371700.014.108294537
GAGGTCA487950.013.9733585
AGGTCAC496450.013.7210016
ACCCGGC85800.013.5330575
TGGGAGG399850.013.5324422
GCATAGG391250.013.4320836
GTTGTCG30100.012.93054426
CACTCTC526950.012.70131510
TCACTCT546100.012.4413549
CTGGGAG481850.012.1022081
Produced by FastQC (version 0.11.5)