FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52595194
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1545600.29386715447803086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT295300.025.57222445
AGACCGC340250.022.5069714
CAGACCG354250.021.693683
GACCGCG353500.021.637915
ACCGCGT354750.021.5870276
AGAACGA353600.021.44952844
CCGCGTT366600.020.8769767
CGCGTTC371650.020.5811948
AATACGG393700.019.13222732
TCAGACC407800.019.0876522
ATACGGA399300.018.9213333
TACGGAG397250.018.89539334
GCGTTCT406200.018.8693859
AAGAACG406250.018.6692443
CAATACG416450.018.22811531
CTCAGAC458150.018.1078741
ACGGAGA419500.017.97193135
CCCAATA439450.017.39091929
CGGAGAG448550.016.73217236
CCTCTCA482400.016.12751219
Produced by FastQC (version 0.11.5)