FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55797606
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36050.012.48940626
TGTCGTT42150.010.63130428
ATATAGC51850.010.4918456
CACCGAA254350.09.7161745
GCACCGA254000.09.53594744
GCTAAGT49650.09.10803714
CGTGGCG120600.08.8313071
TAATCGG38650.08.7306334
GCGTTCG17650.08.66871615
CACCCGG106950.08.6531091
AGGGATC143600.08.617022
TTACGCG12950.08.34381535
CGGCTAA70000.08.29964231
ATAGGGG60700.08.26600738
AATATAG93200.08.2531645
TATAGCT64050.08.2470187
GTCGTTC56600.08.19574629
GTGGCGA130950.08.0921562
ATCGGTT50100.07.94183356
TCAGACG83750.07.87103132
Produced by FastQC (version 0.11.5)