FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54878226
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT856800.15612749581227353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38650.024.7921666
GAACGAT217200.020.7584845
TCGACGA47400.020.7374157
CGACGAG46700.020.2771878
CGGTATA63050.020.26711720
GACCGCG230300.019.6140295
ACCGCGT233750.019.4477356
AGACCGC238050.019.1171864
CCGCGTT241700.018.7986347
GTTCGAC51650.018.6410565
CGCGTTC248300.018.343868
CAGACCG256400.017.9069023
AGAACGA270950.016.79843144
AATACGG278700.016.23256732
TACGGAG277950.016.1064934
ATACGGA281500.016.06311633
TCAGACC302950.015.7048762
GCGTTCT295150.015.5387669
CAATACG297000.015.33084731
AAGAACG306350.014.85419643
Produced by FastQC (version 0.11.5)