FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54878226
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG174200.012.03725838
GTTGTCG34800.012.02459926
GGCATAG170400.011.98800335
AGCTTAG170350.011.83673245
GGGAGGT266200.010.8270663
GGAGGTC252550.010.7529994
GTCACTC253400.010.30925758
GAGCTTA199600.010.22616144
CATAGGG202400.010.09334637
TGGCATA207450.010.04209134
GGTCACT267650.09.9443447
GCATAGG210050.09.77912636
ACTCTCC280650.09.70200911
AGGTCAC281950.09.6553316
GAGGTCA291000.09.4481675
ACCCGGC69150.09.3381115
TAATCGG58700.09.0457314
TGTCGTT49550.08.71737628
TCAGACG86250.08.5822472
GTAACGA57150.08.54373318
Produced by FastQC (version 0.11.5)