FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44825929
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1722810.3843333620592671No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT696540.1553877444458541No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA529030.1180187475869156No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA517620.11547334579501967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA41500.031.98741132
ACGAACG43550.030.48138231
GCCGGCT292950.028.7510721
CCGGCTT321350.027.3059672
AACGCTA333100.026.11988823
ACGCTAC334650.025.94512224
CGCTACC340500.025.56544925
TTCACGC349600.025.5627847
TCACGCT350900.025.2436648
AAAACGC356400.024.55110521
GAAAACG359500.024.38946720
AACGAAC54550.024.2935730
AAACGCT364250.024.02200322
CGGCTTC400800.023.5266593
ACGCTCA384800.022.97892810
GTCCGTT35000.022.1986731
TAAAACG62650.021.61957727
CTACCTC414250.021.1606127
GAACGAG63950.021.00435833
AACGAGA64100.020.92010534
Produced by FastQC (version 0.11.5)