FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44825929
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA507460.1132068004658643No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG492680.10990960165041978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1002.0507452E-517.99868427
GGGAGGT178050.014.1937013
TATGCGT22600.013.13992931
TTATGCG24000.012.93629530
AGCTTAG184750.012.69984145
TCGTAGG8050.012.57582332
TCAGACG110950.012.5145362
GGAGGTC207750.012.2947034
TCGCGAA4500.011.9974540
TATTGCG14650.011.98079917
ATAGGGG191500.011.97018738
ATTAGCG5450.011.97007838
CGTTTCG5150.011.79376744
TCTCGAC42700.011.74831245
TAGCGTC9400.011.72913521
ACTAGCG7900.011.6796966
CCGTACG7900.011.67882316
GGCATAG199650.011.59467435
GTTATCG16900.011.5851097
CAGACTC223300.011.5190153
Produced by FastQC (version 0.11.5)