FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48433486
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT767530.15847093888719882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37500.029.3996076
TCGACGA43750.025.251047
GACCGCG148450.024.7504525
AGACCGC152200.024.2591484
CGGTATA64300.024.17925520
ACCGCGT153200.024.0124056
CGACGAG47750.022.853058
CCGCGTT162650.022.6034017
GAACGAT165950.022.42549945
CGCGTTC164300.022.39018
CAGACCG169700.021.9165733
AATACGG182150.020.27044532
ATACGGA183800.020.07625433
TACGGAG188600.019.45794934
GTTCGAC57700.019.22425
TCAGACC204950.018.707062
AGAACGA201500.018.52506444
CAATACG204450.018.24623531
GTATAGA94800.017.80035822
AACGCTA131850.017.49119823
Produced by FastQC (version 0.11.5)