FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48433486
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG515170.10636649197623314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174700.015.94215345
GGCATAG178700.015.56024135
ATAGGGG185450.015.28484838
GTTGTCG36500.014.97759326
CATAGGG198900.014.00242737
GAGCTTA200600.013.93987844
GTCACTC257650.013.6433578
TGGCATA207850.013.47540134
GCATAGG211150.013.13694436
GGTCACT270050.013.0837197
GGGAGGT296750.012.8823733
ACTCTCC275700.012.80512611
GGAGGTC287150.012.6454834
AGGTCAC289550.012.5601256
TGTCGTT42850.012.44295428
AACTCTA116050.011.88323245
ATATAGC51200.011.8238396
GAGGTCA316750.011.6341515
TATAGCT52850.011.5824297
ATAGCTC77750.011.34232939
Produced by FastQC (version 0.11.5)