FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53029383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1335550.25185094082652254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT259350.024.80052445
ACCGCGT291500.022.1716446
AGACCGC294000.022.0278174
GACCGCG296750.021.7781725
CCGCGTT300150.021.517697
CAGACCG306150.021.3006653
CGCGTTC303850.021.2630718
AGAACGA314150.020.55897344
TTCGACG22100.019.9509966
TTCGGCG110950.019.52559544
AATACGG332550.019.22755432
TCAGACC344450.019.1936132
ATACGGA335450.019.0607233
TACGGAG343600.018.54276534
TCGGCGC116750.018.44139545
GCGTTCT353850.018.3093999
ACGTTCG35400.017.602626
ACGGAGA377000.016.9408335
CTCAGAC431400.016.873411
CCCAATA385550.016.82373829
Produced by FastQC (version 0.11.5)