FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53029383
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG561340.10585452219951343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC128850.016.6597335
AGCTTAG182950.015.452785545
ACGGACA139600.015.13286718
TCACCCG148250.014.7372463
CGGACAC143750.014.5746313
CGGACAG149450.014.09032319
GGCATAG198350.014.007936535
CCGGACA157400.013.68243212
ATAGCTC150600.013.49056239
CACCCGG163250.013.4660854
GTCACTC273050.013.31858
ACACGGA158050.013.23894216
GAGCTTA216700.013.13556844
GGTCACT276450.013.11399657
CACGGAC162050.013.02284617
GACACGG161200.012.85590915
GCCCGGA170150.012.81586510
CATAGGG221250.012.74138437
TAGCTCT165650.012.26551240
TGGCATA232750.012.08258134
Produced by FastQC (version 0.11.5)