FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48222840
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23800.021.3651376
AGACCGC101850.018.4462744
ACCGCGT102500.018.2411146
GACCGCG106450.017.5434765
CGGTATA38900.017.00481420
TCGACGA31450.016.5974567
CCGCGTT113950.016.4279427
CGCGTTC116300.016.0766498
CAGACCG122050.015.8173133
AACGCTA137650.015.02150523
ACGCTAC138100.014.93997424
GTCCGTT36900.014.8211541
GAACGAT127900.014.81220745
CCCGTAT28250.014.5792351
CGCTACC146950.014.04022125
AATACGG135350.013.9808232
GTTAGTT111850.013.77952344
ATACGGA137400.013.75586633
CGACGAG36000.013.7497368
TACGGAG139600.013.36179934
Produced by FastQC (version 0.11.5)