FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48222840
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161900.014.93839745
GGCATAG166650.014.3238935
ATAGGGG174100.013.87869938
CATAGGG186200.012.8802437
GTCACTC250350.012.8425788
GAGCTTA191200.012.74326544
GGGAGGT276900.012.6101033
GGTCACT258000.012.4366517
GGAGGTC265050.012.4260274
ACTCTCC266150.012.19835211
TGGCATA198350.012.1707834
GCATAGG196750.012.12108836
GTTGTCG27200.011.99340226
GAGGTCA310900.010.9985235
AGGTCAC318750.010.6322626
TAGGGGA297650.010.59693939
CACTCTC306550.010.532145510
TCACTCT322400.010.3074939
TAGCGTA10150.09.75288437
CGTAGGG77700.09.41041637
Produced by FastQC (version 0.11.5)