FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50004534
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT731540.14629473399352147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC140200.025.1597884
ACCGCGT146600.024.0921176
CAGACCG150550.023.6847653
GACCGCG150600.023.3923875
CCGCGTT155200.022.771617
GAACGAT152850.022.68220345
CGCGTTC157900.022.382238
AATACGG174100.020.10745232
AGAACGA177450.019.58697944
TACGGAG178750.019.5466234
ATACGGA180650.019.31612633
TCAGACC193900.018.889052
GCGTTCT192350.018.4437319
ACGGAGA204750.017.13026435
CAATACG207250.017.08637231
CTCAGAC246250.016.9392661
AAGAACG207400.016.92118543
CCCAATA219750.016.12415929
AACGCTA71500.016.01478423
ACGCTAC71600.015.92967324
Produced by FastQC (version 0.11.5)