FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50004534
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG105000.012.66242235
AGCTTAG107150.012.49769245
ACCCGGC95300.012.1410825
CATAGGG111500.012.10488537
TCACCCG97200.011.9969683
ATAGGGG115850.011.88313438
GCATAGG119900.011.31367636
ACGGACA101900.011.28073918
GCGTAGG57100.010.83433236
GGCGTAG58250.010.77523935
ATAGCTC96300.010.74480339
GAGCTTA127550.010.7400344
CGTAGGG58550.010.64234137
GGGAGGT233650.010.59826853
ACACGGA109150.010.26427216
GACACGG110400.010.189054515
CACCCGG114300.010.1627054
TAGCTCT103850.010.09334340
TGGCATA134200.010.07556134
ACTCTCC213000.09.72953411
Produced by FastQC (version 0.11.5)