FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62990111
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1289920.20478135052024277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT240450.027.76493345
ACCGCGT286050.023.4822866
GACCGCG288600.023.2922465
CCGCGTT291200.023.0747187
CGCGTTC292700.022.933418
AGACCGC293900.022.8875314
AGAACGA293950.022.81363344
CAGACCG317750.021.3326233
AATACGG318400.020.92302132
ATACGGA323000.020.63184733
TACGGAG323950.020.49574734
AAGAACG340600.019.6369143
CAATACG348600.019.19474231
TCAGACC362800.018.9754242
AACGCTA56700.018.96701823
GCGTTCT357150.018.9083029
ACGGAGA364850.018.2825935
ACGCTAC60350.018.11812424
CGGAGAG390900.017.0816236
CCCAATA405250.016.72808529
Produced by FastQC (version 0.11.5)