FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62990111
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161450.014.19910945
GGCATAG168300.013.48755235
ATAGGGG176750.012.98285838
CATAGGG181400.012.41438737
GCATAGG191550.011.81522236
ACCCGGC110300.011.5263315
GTCACTC269150.011.4800828
GAGCTTA203150.011.44122944
GGTCACT269050.011.2082617
AGGTCAC291300.010.7533596
TGGCATA214300.010.66591134
ATAGCTC117050.010.57123339
ACGGACA118600.010.56566718
ACTCTCC303550.010.40179611
GGGAGGT333250.010.3642723
TAGGGGA295800.010.22959639
TCACCCG140300.09.9433923
GGAGGTC322400.09.8269064
GAGGTCA326650.09.8232455
CGGACAG130150.09.71441219
Produced by FastQC (version 0.11.5)