FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46308122
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT793680.1713911006799196No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG687720.1485095854243452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT152450.025.8366456
AGACCGC153900.025.6939544
GACCGCG154600.025.4611575
CAGACCG164050.024.2415313
CCGCGTT164400.023.931257
CGCGTTC172000.022.886858
AACGCTA169400.022.85303523
ACGCTAC169800.022.73296424
CGCTACC176900.021.7569825
TTCGACG21450.021.0788126
TCAGACC197200.020.6573962
AAAACGC190750.020.33055121
GAACGAT198250.019.69695545
TCACGCT206450.019.4600098
GAAAACG200300.019.316320
AATACGG202000.019.28967932
AAACGCT203300.019.10871122
ATACGGA205400.019.03669233
TACGGAG207350.018.7817134
GCGTTCT216400.018.2949779
Produced by FastQC (version 0.11.5)