FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40407462
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA123200.019.20349323
ACGCTAC126350.018.81422424
CGCTACC131000.018.12987725
TCACGCT146400.017.0585178
AAAACGC142050.016.77885221
CTACACG50550.016.36452545
CTCGGTT84700.016.3469431
GAAAACG147200.016.25132220
ACGCTCA152600.016.2335310
CCGGCTT178300.015.8925252
TTCACGC157550.015.8085777
TTCGACG15900.015.6503126
AAACGCT159350.014.91707622
CGGTTTT87650.014.834513
TCGGTTT92000.014.7422822
GTCCGTT28750.014.6742121
CACGCTC176850.014.2737289
GCCGGCT176600.014.2572891
TCGTTAC19050.014.12161944
CGGCTTC220500.013.7049813
Produced by FastQC (version 0.11.5)