FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40407462
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG523260.1294958837058363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG183650.014.1732945
ATAGGGG192750.013.64420938
GGCATAG190550.013.542047535
GGAGGTC252550.012.6516024
GAGCTTA210900.012.44865744
GGGAGGT268950.012.2144233
TGGCATA214000.012.12122334
GTTGTCG22300.012.10708326
GGTCACT265050.011.8090857
ACTCTCC267600.011.77280111
GTCACTC266100.011.7625038
CATAGGG237400.010.94534737
TAATCGG36800.010.7002724
GCATAGG246400.010.490787536
TCACTCT305950.010.4734799
CACTCTC300700.010.46183510
ATAATCG40100.010.2121853
GAGGTCA327800.09.9121695
ATCGGTT41450.09.7715346
GTCGTAA19600.09.75649434
Produced by FastQC (version 0.11.5)