FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41093682
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2225230.5415017325534374No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1864790.4537899524311304No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1571850.38250405500290774No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1540710.3749262477867036No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC940590.22888920004783217No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC885750.2155440829079273No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG805590.19603743465966372No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA750780.18269961791206735No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG696770.16955647829269715No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT560920.1364978684557884No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT557080.1355634182403027No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT495660.1206170817207375No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG452140.11002664594523313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG17250.022.5634466
TCACGCT403500.021.160058
AGACCGC537650.020.9165174
CAGACCG539350.020.8632343
GTTAGTT358300.020.837344
GCCGGCT383250.020.2524091
GAACGAT525550.020.11885545
GTTTTCG414100.020.11063245
TTCACGC441350.019.579867
CGTGGTT426500.019.54131141
ACCGCGT578250.019.4615086
AACGCTA433000.019.38097823
GACCGCG580900.019.3704095
ACGCTAC434450.019.28522124
CGGTATA21950.019.1681820
TCGTGGT434750.019.1486240
GCGTTCT588500.019.1148749
CCGCGTT590550.019.0714027
TTCGGCG491000.019.00484744
CGCGTTC593050.018.9872118
Produced by FastQC (version 0.11.5)