FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50664949
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT929390.18343845564711808No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG736810.14542795651486792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC205250.026.7595334
GACCGCG206000.026.5965715
ACCGCGT211450.026.0172396
CAGACCG213000.025.8599363
CCGCGTT221050.024.8262147
CGCGTTC228150.024.0824748
AACGCTA179400.022.33710323
ACGCTAC181850.022.0361224
GAACGAT248100.021.97371145
TCAGACC253900.021.9096052
AATACGG217600.021.84817932
CGCTACC186150.021.30948325
ATACGGA264600.020.74779933
TACGGAG265150.020.58596434
GCGTTCT270700.020.3884169
AAAACGC198300.020.33290121
CAATACG236500.020.26394331
AGAACGA271050.020.15468644
TTCGGCG94400.019.44889544
GAAAACG214450.018.82263820
Produced by FastQC (version 0.11.5)