FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51287884
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2294770.44742926029079305No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG771320.1503902949086377No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA553170.10785588268761488No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG535170.10434628186259351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT428050.028.46665245
TTCGACG36950.025.8133956
AGACCGC515900.023.9174064
GACCGCG511150.023.9151215
ACCGCGT515800.023.7688876
AGAACGA519150.023.54314644
CAGACCG528400.023.49243
CCGCGTT528350.023.20437
CGCGTTC533350.022.999428
TCAGACC569100.022.2155082
AAGAACG574150.021.25985543
GCGTTCT580600.021.1859179
AATACGG583500.020.8980732
ATACGGA583050.020.8769333
CTCAGAC641200.020.7779241
TACGGAG582100.020.77065534
CGACGAG45400.020.6125248
CAATACG605250.020.21919631
CGTTCTC636700.019.72552510
ACGGAGA617750.019.63419735
Produced by FastQC (version 0.11.5)