FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51287884
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA712010.13882616019019228No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG615340.11997765398159145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG45850.016.58717226
ATATAGC60550.013.7071456
GTCGTTC58550.012.9899329
TGTCGTT60300.012.91147228
GGGAGGT278400.012.435983
GCTAAGT61050.012.42193414
AGCTTAG193150.012.405292545
GGCATAG197450.012.23904835
ATAGGGG201200.012.21174738
GTCACTC272950.012.1474168
TATAGCT70750.012.0807677
GGAGGTC282300.011.871964
GGTCACT283600.011.5000337
TCGTTCC67800.011.41708130
GAGCTTA222750.010.99050344
ACTCTCC304250.010.95104211
AGGTCAC312850.010.906476
TGGCATA231050.010.57683534
CATAGGG232000.010.47399937
CAATATA88400.010.3559334
Produced by FastQC (version 0.11.5)