FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51888168
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT134850.018.5534536
AGACCGC136900.018.3413664
GAACGAT140750.017.92080145
CGCGTTC147750.016.9182648
CCGCGTT148150.016.9030277
CAGACCG149750.016.902723
GACCGCG152000.016.5340985
AGAACGA168000.015.08084144
GTCCGTT35300.014.6673261
ACGCTAC158000.014.51068824
AACGCTA157950.014.45820623
ACGTTCG17550.014.358596
AATACGG173000.014.358191532
TACGGAG175100.014.25023834
ATACGGA176950.014.19026233
CCCGTAT34450.014.1143961
TCAGACC188350.013.868852
CGCTACC166550.013.698235525
TCACGCT179150.013.4380488
TCGGCGC56050.013.08706345
Produced by FastQC (version 0.11.5)