FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51888168
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4149370.7996755638009806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC82050.011.6820935
AGCTTAG86900.011.23879145
TCACCCG94250.011.0066643
GGCATAG85950.010.9160735
GTTGTCG23450.010.84102526
CATAGGG89400.010.44382837
GCATAGG94500.09.85717136
ATAGGGG98600.09.83428438
CACCGAA210950.09.80360145
ACGGAAC71150.09.8032046
ACGGACA98900.09.78216218
CACCCGG101250.09.7775964
CCGGACA100650.09.52344112
ATAGCTC98900.09.3723239
CGGACAG106250.09.04196619
TGGCATA107200.09.02494934
GAGCTTA109900.08.96858544
GCGTAGG46050.08.9406239
TCGCGCT33400.08.82621445
GCACCGA234150.08.80336844
Produced by FastQC (version 0.11.5)