FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54076560
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1979400.36603659700247204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT345450.027.75294145
AGACCGC415750.023.3838184
AGAACGA414950.023.13332444
CAGACCG429100.022.7875143
GACCGCG428200.022.6776335
ACCGCGT430300.022.6300166
TTCGACG28200.022.0155016
CCGCGTT444950.021.8899777
CGCGTTC451350.021.57468
ATACGGA463750.020.68308433
AATACGG463950.020.67970732
TCAGACC483250.020.5618132
AAGAACG467150.020.56140943
TACGGAG467650.020.43327534
CAATACG481050.019.99984231
GCGTTCT490350.019.8770149
ACGGAGA499800.019.17670235
CCCAATA509600.018.95454429
CTCAGAC564350.018.6088261
CCAATAC533950.018.2207330
Produced by FastQC (version 0.11.5)