FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54076560
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG684110.12650767726349457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33050.016.74643926
AGCTTAG200800.016.71814545
GGCATAG226350.014.75935735
ATAGGGG230200.014.68829938
GTCACTC321500.014.0557368
GAGCTTA245950.013.75038244
GGTCACT325400.013.6787467
GGGAGGT345150.013.6642313
CATAGGG258050.012.96331737
TGGCATA262800.012.87510534
TGTCGTT43650.012.78280828
ACTCTCC355500.012.69250311
AGGTCAC364850.012.6062996
GCATAGG271600.012.35804336
GGAGGTC376650.012.2585184
ATATAGC52250.012.1876846
GAGGTCA385250.012.13165
TCACTCT407100.011.271689
CACTCTC413000.010.87089610
TAGGGGA414150.010.70648339
Produced by FastQC (version 0.11.5)