FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49013849
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG595310.12145750887672584No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT504000.102828080284003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26950.021.5374626
AACGCTA185700.018.09894623
ACGCTAC187400.018.05480624
GTCCGTT36050.017.8737811
TCACGCT199950.017.4728858
CGCTACC194500.017.26850725
CGACGAG32700.016.7863648
TCGACGA34250.016.6841017
AAAACGC204250.016.57635521
AGACCGC56500.016.4151294
ACGTTCG19700.016.3306486
GCCGGCT216450.016.1231351
ACCGCGT57300.016.0584016
CGAACGA44850.015.95090932
GAAAACG215700.015.69643120
ACGCTCA226900.015.68507410
CCGGCTT242900.015.4681722
CCCGTAT30600.015.4615691
GACCGCG59450.015.373225
TTCACGC231900.015.2213037
Produced by FastQC (version 0.11.5)