FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46927057
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG693590.14780172555888174No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT652200.13898165401678608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT139500.023.9664446
AGACCGC143650.023.4933344
GACCGCG142750.023.3735165
TTCGACG23700.023.2582366
CCGCGTT149800.022.2885137
CAGACCG154250.022.0830843
CGCGTTC153850.021.6432888
AACGCTA198750.020.14985323
ACGCTAC199450.020.11297624
CGCTACC207750.019.25527625
TCACGCT226500.018.316898
GAACGAT183350.018.27163545
AAAACGC225400.017.82734121
TCGACGA32050.017.690157
CGGTATA46650.017.5553220
TCAGACC201300.017.4468962
GCCGGCT224450.017.3688491
ACGCTCA244550.017.19493910
GCGTTCT194350.017.167849
CGACGAG34350.016.833158
Produced by FastQC (version 0.11.5)