FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46927057
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG126000.013.12785735
AGCTTAG126850.012.93085145
ATAGGGG129300.012.91794938
CATAGGG135450.012.131989537
GCATAGG143650.011.48590536
GTCACTC187550.011.4193638
GTTGTCG23850.011.41386926
GAGCTTA149650.011.20154544
TAGCGTA8150.011.04817637
TCAGACG70050.010.9842772
GGTCACT196100.010.6692497
TGGCATA157550.010.65370734
GGGAGGT228950.010.4751973
GGAGGTC216050.010.4029364
ACTCTCC212850.010.18883311
AGGGATC122550.09.9870922
AGGTCAC228900.09.6907686
GAGGTCA239200.09.4428765
CGTGGCG120300.09.2982981
TAATCGG42400.09.0204414
Produced by FastQC (version 0.11.5)